Watershed – Optimized Crystal Harvesting
Prevent loss or damage to the crystals that you’ve spent time growing and optimizing. The Watershed™ system protects drops from drying out during crystals harvesting, ensuring you obtain the best possible diffraction quality. Precisely control the humidity around your crystallization drops whenever you are harvesting crystals.
Product Information
Simplify your crystal mounting
Watershed™ will simplify your crystal mounting allowing you to get the best possible diffraction which in turn allows you to optimize your crystal harvesting and post-growth treatment protocols. Additional benefits of using Watershed™ are:
- Increase drop working times and prevent dried out drops
- Reduce protein precipitation and degradation
- Harvest more crystals from each drop
- Reduce crystal non-isomorphism
- Used controlled dehydration to improve diffraction
- Use with cover slips, glass slides, crystallization plates, or your home X-ray source
Controlled Humidity Environment
Watershed™ provides a controlled humidity environment for a wide range of common sample preparation tasks, including:
- Separating crystals from each other, from precipitate, and from protein skins that may form over crystallization drops
- Soaking crystals in solutions containing cryoprotectants, heavy atoms or ligands
- Harvesting crystals from drops onto X-ray sample mounts
- Blotting or wicking away excess liquid from crystals to reduce background X-ray scatter and increase sample cooling rates during plunge cooling
- Placing crystals in MiTeGen’s MicroRT capillaries or glass capillaries for room temperature data collection
- Adjusting the hydration of crystals to a desired relative humidity, which may be different from their as-grown relative humidity, to modify protein structure or optimize diffraction properties
Watershed is already used at several facilities
- UCSF Macromolecular Structure Group
- Diamond Light Source XChem Pipeline
- The CUNY ASRC Macromolecular Crystallization Facility
- Hauptman-Woodward Medical Research Institute - University of Buffalo
- Max Planck Institute for Developmental Biology
- And more!
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