SYBR® Green Fluorescent DNA Stain
DNA intercalation dye for real-time PCR analysis
For in vitro use only!
- Shipping: shipped on gel packs
- Storage Conditions: store at -20 °C
store dark - Shelf Life: 12 months
- Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
- Color: orange
- Concentration: 100 μM
- Spectroscopic Properties: λexc 495 nm, λem 520 nm (bound to DNA)
- Manufacturers SKU: PCR-378
- MiTeGen SKU: M-PCR-378
Product Information
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® Green or FAM on the detection instrument.
| final SYBR® Green concentration | 20 μl PCR assay | 50 μl PCR assay |
| 0.5 μM | 0.1 μl | 0.25 μl |
| 1.0 μM | 0.2 μl | 0.50 μl |
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.