ROX Reference Dye
Reference dye for fluorescence signal normalization
For in vitro use only!
- Shipping: shipped on gel packs
- Storage Conditions: store at -20 °C
store dark - Shelf Life: 12 months
- Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20.
- Color: pink
- Concentration: 25 μM (50x conc.)
- Spectroscopic Properties: λexc 576 nm, λem 601 nm
- Manufacturers SKU: PCR-351
- MiTeGen SKU: M-PCR-351
Product Information
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
Preparation of the qPCR master mix:
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use. Add ROX reference dye to your PCR assay as indicated below.
| final ROX concentration |
20 μl PCR assay | 50 μl PCR assay |
| low ROX assay 50 nM |
0.04 μl | 0.1 μl |
| high ROX assay 500 nM |
0.4 μl | 1.0 μl |