Lysozyme – Solution

Crystallization grade model protein

Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase

For in vitro use only!

  • Shipping: shipped at ambient temperature
  • Storage Conditions: store at 4
    do not freeze
  • Shelf Life: 12 months
  • Molecular Weight: 14.3 kDa (single chain)
  • CAS#: 12650-88-3
  • EC number: 235-747-3
  • Form: aqueous solution
  • Concentration: 20 mg/ml
  • pH: 3.5 (H2O, 20 °C)
  • Solubility: 300 g/l in water
  • Activity: 50 kU/mg (Micrococcus luteus, FIP-Standard; pH 7.0; 25 °C)
  • Manufacturers SKU: CO-401
  • MiTeGen SKU: M-CO-401
Learn More About This Product

$107.00

SKU: M-CO-401 Category:

Product Information

Description:
Lysozyme is a glycosidase which hydrolyzes the ß 1,4 glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the bacterial peptidoglycan[1].

Source: Chicken egg white

Usage:
Crystals can be grown using the sitting drop, hanging drop or microbatch method.
Following crystallization conditions are suggested for lysozyme crystal growth:

  • 0.7 - 1.5 M Sodium Chloride, 100 mM Sodium Acetate pH 4.0 - 4.8[2]
  • 10 % w/v Sodium Chloride, 5% v/v Propanol[2]
  • 0.44 M Sodium Nitrate, 100 mM Sodium Acetate pH 4.6[2]

Applications:
Lysozyme can be utilized as model protein in crystallization experiments and X-ray structure analysis.

Selected References:
[1] Rye et al. (2000) Glycosidase mechanisms. Curr. Opin. Chem. Biol. 4:573.
[2] Gilliland et al. (2002) The Biological Macromolecule Crystallization Database: crystallization procedures and strategies. Acta Cryst. D 58:916.