Lysozyme – Solution
Crystallization grade model protein
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase
For in vitro use only!
- Shipping: shipped at ambient temperature
- Storage Conditions: store at 4
do not freeze - Shelf Life: 12 months
- Molecular Weight: 14.3 kDa (single chain)
- CAS#: 12650-88-3
- EC number: 235-747-3
- Form: aqueous solution
- Concentration: 20 mg/ml
- pH: 3.5 (H2O, 20 °C)
- Solubility: 300 g/l in water
- Activity: 50 kU/mg (Micrococcus luteus, FIP-Standard; pH 7.0; 25 °C)
- Manufacturers SKU: CO-401
- MiTeGen SKU: M-CO-401
Product Information
Description:
Lysozyme is a glycosidase which hydrolyzes the ß 1,4 glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the bacterial peptidoglycan[1].
Source: Chicken egg white
Usage:
Crystals can be grown using the sitting drop, hanging drop or microbatch method.
Following crystallization conditions are suggested for lysozyme crystal growth:
- 0.7 - 1.5 M Sodium Chloride, 100 mM Sodium Acetate pH 4.0 - 4.8[2]
- 10 % w/v Sodium Chloride, 5% v/v Propanol[2]
- 0.44 M Sodium Nitrate, 100 mM Sodium Acetate pH 4.6[2]
Applications:
Lysozyme can be utilized as model protein in crystallization experiments and X-ray structure analysis.
Selected References:
[1] Rye et al. (2000) Glycosidase mechanisms. Curr. Opin. Chem. Biol. 4:573.
[2] Gilliland et al. (2002) The Biological Macromolecule Crystallization Database: crystallization procedures and strategies. Acta Cryst. D 58:916.