LEXSY BHI – Powder Media Kit
Sterile, brain-heart infusion-based medium. Recommended for transfection and strain maintenance.
Three-component Kit for preparation of complex Leishmania cultivation medium.
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
- Shipping: shipped on gel packs
- Storage Conditions: Store components as indicated on individual labels
- Shelf Life: 12 months
- Manufacturers SKU: ML-412L, ML-412S, ML-412XL, ML-412XXL
- MiTeGen SKU: M-ML-412L, M-ML-412S, M-ML-412XL, M-ML-412XXL
Product Information
- Applications: For the growth of LEXSY hosts P10 (M-LT-101) and T7-TR (M-LT-110).
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Content:
LEXSY BHI, liquid
Hemin stock solution, 500x (M-ML-108L):
0,25% solution of Hemin in 30% Triethanolamine, sterilePen-Strep stock solution, 200x (M-ML-105L):
10.000 U/ml of penicillin G sodium salt and 10.000 μg/ml of streptomycin sulfate in 0.85 % saline, sterile
| Cat. No. | Content | Amount |
| ML-412S | 37 g powder 2 ml Hemin 5 ml Pen-Strep |
for 1 l medium |
| ML-412L | 185 g powder 10 ml Hemin 25 ml Pen-Strep |
for 5 l medium |
| ML-412XL | 370 g powder 20 ml Hemin 50 ml Pen-Strep |
for 10 l medium |
| ML-412XXL | 1850 g powder 5x 20 ml Hemin 250 ml Pen-Strep |
for 50 l medium |
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Storage conditions:
LEXSY BHI, liquid:
Store at room temperature
Stable for 12 months
Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month
Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month
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Preparation of complete Leishmania cultivation medium:
Dissolve 37 g/l LEXSY BHI powder in deionized water and autoclave for 15 min at 121 °C. Control temperature and time with a reference with the same volume, since overexposition leads to de-composition of ingredients and a dark brown color of the medium, which adversely affects growth of LEXSY strains. Proper autoclaved medium is of amber color. Store medium at room temperature before addition of Hemin and Pen-Strep (stable for 12 month). As an alternative, the medium can also be sterile filtered.
Add to 1 l LEXSY BHI:
5.0 ml Pen-Strep solution
2.0 ml Hemin stock solution to a final concentration of 5 μg/ml
Final pH = 7.6 ± 0.2
Store in the dark at 4 °C.
Completed medium is useable for up to 2 weeks. If the completed medium is to be used after this period, appropriate amounts of Hemin and Pen-Strep have to be re-added.