MiTeGen
X06DA-PXIII Beamline at SLS, Cryo-EM Pucks, CombiPucks, ACA Meeting Preview, & more!
May 2017
BEAMLINE SPOTLIGHT
X06DA-PXIII – A Phasing Beamline at the Swiss Light Source
A Versatile Beamline for Macromolecular Crystallography
X06DA-PXIII SLS endstation with a PRIGo goniometer & a PILATUS 2M-F detector. (Photo: PSI/Markus Fischer)
X06DA-PXIII [1] at the Swiss Light Source, celebrating its 1,000 PDB entries, is among the most productive macromolecular crystallography beamlines worldwide. X06DA receives light from a 2.9 T superbend magnet, which provides beam performance comparable to undulators, but with very high stability. Therefore, X06DA is not only compatible with high-throughput measurements – well appreciated by 1/3 industrial usage – but is also highly optimized for experimental phasing experiments. Beyond its remarkably fast and stable optics, X06DA offers unique hardware [2] and software tools for advanced phasing data collection protocols [3], which improve both the quality of the anomalous signal and the efficiency with which it is being measured. The current increase in number of structures solved by native SAD (single-wavelength anomalous dispersion) reflects the routine use of a simple and fast data collection protocol developed at X06DA [4], which has proven effective on highly challenging cases [5]. In situ X-ray diffraction screening of crystallization plates, available during users beamtime with a fast switching (two minutes) between in situ and cryogenic mode, further expands the capabilities of the beamline and is available as a service from the integrated crystallization facility [6].
View the X06DA-PXIII Beamline Guide Page
View GIF of X06DA-PXIII in Action
References
1. http://www.psi.ch/sls/pxiii/
2. Waltersperger S., Olieric V., Pradervand C., Glettig W., Salathe M., Fuchs M.R., Curtin A., Wang X., Ebner S., Panepucci E., Weinert T., Schulze-Briese C., Wang M. (2015) PRIGo: a new multi-axis goniometer for macromolecular crystallography. Journal of Synchrotron Radiation, 22(Pt 4):895- 900. http://dx.doi.org/10.1107/S1600577515005354
3. Finke A.D., Panepucci E., Vonrhein C., Wang M., Bricogne G., Olieric V. (2016) Advanced Crystallographic Data collection protocols for experimental phasing. Methods in Molecular Biology, 1320:175-91. http://dx.doi.org/10.1007/978-1-4939-2763-0_11
4. Weinert T., Olieric V., Waltersperger S., Panepucci E., Chen L., Zhang H., Zhou D., Rose J., Ebihara A., Kuramitsu S., Li D., Howe N., Schnapp G., Pautsch A., Bargsten K., Prota A., Surana P., Kottur J., Nair D., Basilico F., Cecatiello V., Pasqualato S., Boland A., Weichenrieder O., Wang B.C., Steinmetz M., Caffrey M., Wang M. (2015) Fast native SAD phasing for routine macromolecular structure determination. Nature Methods, 12(2):131-3. http://dx.doi.org/10.1038/nmeth.3211
5. Olieric V., Weinert T., Finke A.D., Anders C., Li D., Olieric N., Borca C, Steinmetz M.O., Caffrey M., Jinek M. and Wang M. (2016) Data collection strategy for challenging native SAD phasing. Acta Cryst. D72, 421-429. http://dx.doi.org/10.1107/S2059798315024110
6. Bingel-Erlenmeyer B., Olieric V., Grimshaw J.P.A., Gabadinho J., Wang X., Ebner S.G., Isenegger A., Schneider R., Schneider J., Glettig W., Pradervand C., Panepucci E.H., Tomizaki T., Wang M., Schulze- Briese C. (2011) The SLS crystallization platform at beam-line X06DA – a fully automated pipeline enabling in situ X-ray diffraction screening. Crystal Growth & Design, 11 (4), pp 916–923. https://pubs.acs.org/doi/abs/10.1021/cg101375j
CRYO-EMTM PUCKS
Organized Storage and Transport of Cryo-EM Grid Boxes
Simply and Securely Know Where Each Grid Box Is
Simplify how you store and transport your cryogenically cooled Cryo-EM specimen grids. Each puck holds up to 24 round Cryo Grid Boxes (96 grids). A custom serial number on each puck allow easy sample tracking. An 8 puck capacity storage cane is compatible with standard refrigerators, such as the HC34. The compatible shelved shipping cane holds 7 pucks and can be used with a CX100 dryshipper.
Safely Store and Ship Specimen Grids
COMBIPUCKSTM
Eliminate Transferring Crystals from Cryovials to Uni-Pucks
Many labs are filled with both cryovials and Universal Pucks (Uni-Pucks). When cryocooling your crystals, it may be hard to predict which storage system will be required once your beam-time rolls around. The CombiPuck was designed so that you don’t have to know. The CombiPuck accepts samples cryocooled into SPINE standard cryovials in a form factor compatible with Uni-Puck handling robots.
SCHEDULE YOUR ACCESS – NANUQTM
Come Cryocool Your Crystals, Simplify Cryoprotection and Eliminate Ice
(Users from the McKenna Lab, University of Florida, Prepare Samples for Plunge Cooling Tests at MiTeGen’s NAF)
The NANUQ™ Access Facility (NAF) is located at MiTeGen’s headquarters in Ithaca, NY, USA, just a few miles away from the CHESS synchrotron beamlines. The facility and support services enable crystallographers to access state-of-the-art equipment that will optimize the cryocooling of their crystals, while reducing or eliminating the need for cryoprotectants. The laboratory facility includes a NANUQ™ cryocooling device, liquid nitrogen supplies and accessories, a Carl Zeiss Discovery V8 stereo microscope outfitted for crystallography, and all the required tools and accessories for controlled, reproducible cryocooling into the Universal V1 Pucks (Uni-pucks). MiTeGen personnel are on site to directly assist in ensuring the success of NAF users.
UPCOMING MEETING: ACA 2017
See you this week at ACA 2017 in New Orleans!
We look forward to seeing many of you this year at ACA in New Orleans. Stop by our booth to see what is new, such as our Cryo-EM Pucks, the NANUQ™ Access Facility (NAF) and much more.
RECEIVE YOUR CRYSTALLOGRAPHY 2017 CATALOG :
Have an idea for a new tool or product, need us to answer a question, or have a comment on our existing products?