BEAMLINE GUIDE
SPring–8 BL26B2
Super Photon ring-8 GeV
Japan
SPACE Automounter
BL26B1 & B2 have been developed as RIKEN Structural Genomics Beamline I & II for high throughput protein crystallography (HTPX). The function of the beamline is to collect diffraction data of a large number of protein crystals for structural analysis. In the experimental hutch, an automatic sample changer developed at BL26 is installed to improve the throughput of the experiment. Cooperating with the sample changer, the beamline scheduling software executes the successive data collections without any user intervention. The beamline design is based on the SPring-8 standard optics for the bending magnet, which is simple and remote controllable. Two type X-ray detectors (IP and CCD) can be automatically switched according to the experimental schedule.1
Automated Systems at BL26B2:
“To achieve high-throughput protein crystallography, we have developed two special components: an automated sample changer robot, SPACE (SPring-8 precise auto-matic cryo-sample exchanger) (Ueno, Hirose et al., 2004), which can change up to 100 crystals in a diffractometer, and the beamline control software BSS (beamline scheduling software) (Ueno et al., 2005), which can perform successive data collection by controlling beamline devices, including SPACE, and managing the data collection schedule. The combination of SPACE and BSS enables unmanned overnight data collection and allows the beamline to operate with high efficiency.” 2
Contacts
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Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
| Recommended | Accepted* | |
|---|---|---|
| Puck Style | Uni-Puck | Uni-Puck, ALS, ACTOR†, SPACE tray |
| Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
| Rod Length | 18mm/SPINE | 18mm/SPINE |
| Base Style | B1A-Reusable | B1A-Reusable, B1-R, B3-R, B3S-R, B5-R, SPACE pin |
| *Robotic Sample Handling, Aina Cohen. Retrieved December 9, 2013.
†To purchase ACTOR pucks, please contact us. |
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Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B5-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
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Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description