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Learn Beamline Guides SLSPSI X06DA - PXIII

BEAMLINE GUIDE
SLSPSI X06DA – PXIII

Swiss Light Source @ Paul Scherrer Institut
Switzerland
CATS Automounter

X06DA (PXIII) is the third macromolecular crystallography beamline at the Swiss Light Source. It was funded by a partnership between the Paul Scherrer Institut and Swiss and international pharmaceutical companies. The beamline receives light from a 2.9 T superbend magnet. The optical design results in a sub 80 microns x 45 microns focused beam at the sample position with a total photon flux comparable to an undulator beamline. With both the PILATUS 2M-F detector and the multi-axis PRIGo goniometer, the setup is well suited for experimental phasing experiments, in particular native SAD. The experimental station offers both efficient manual mounting with a mini-hutch design and automatic robotic sample mounting. The robot also enables In situ X-ray diffraction screening. The switch to this mode of operation only takes 2 min. A crystallization facility, directly adjacent to the experimental mini-hutch, further expands the capabilities of X06DA beamline.1

Contacts

Vincent Olieric

Justyna Wojdyla

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Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style SPINE SPINE
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B5-Reusable B5-R, B-5
*Sample Changer at X06DA. Retrived November 19, 2013.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

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