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BEAMLINE GUIDE
RRCAT PX-BL21

Raja Ramanna Centre for Advanced Technology
India

The protein crystallography (PX-BL21) beamline is installed on 1.5 Tesla bending magnet source (port no. 21; 10o port) of Indus-2 synchrotron. This beamline has been designed for macromolecular crystallography i.e. diffraction studies on single crystals of protein, DNA and their complexes. It has also been designed to perform high-pressure studies using single crystal and powder diffraction experiments. The beamline can be tuned to desired energy in the range between 5 to 20 keV (with bandwidth of ~1 eV) corresponding to wavelength of 2.48– 0.62 Å. The beamline is equipped to perform Single- and Multi- wavelength anomalous diffraction experiments (SAD, MAD) by fine-tuning energy of beam with required energy resolution. The typical time for data acquisition is about 10 to 60 sec per frame depending on the crystal quality and synchrotron beam current. The beamline is associated with a well-equipped biochemical laboratory and cold rooms to grow, cryo-soak and cryo-freeze the protein crystals. 1

Contacts

Anshawni Kumar

Dr. Biplab Ghosh

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Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted
Puck Style SPINE SPINE
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B5-R B5-R

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

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