BEAMLINE GUIDE
EMBL P13
European Molecular Biology Laboratory
Hamburg, Germany
P13 offers micro-beam conditions with a beam size of 30 x 20 micron2 (H x V) and divergence below 0.15 mrad. Typical crystal lifetimes are <1 min. for the focused beam (5 x 1012 ph/sec at 12 keV). By adjusting the shape of the adaptive KB-mirrors, the full beam can be defocused to a size of 150 x 70 micron2 at the sample position. Apertures can be used to collimate the beam down to a diameter of 10 micron. The beamline is tunable between 4.5 and 17.5 keV (2.7 Å to 0.7 Å).1
Contacts
Guillaume Pompidor
Thomas R. Schneider
Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
| Recommended | Accepted* | |
|---|---|---|
| Puck Style | SPINE | SPINE |
| Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
| Rod Length | 18mm/SPINE | 18mm/SPINE |
| Base Style | B1A-Reusable | B1A-Reusable, B1-R, B3-R, B3S-R, B5-R |
| *Crystal Mounts Retrieved September 18, 2017. | ||
Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B5-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
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Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description