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Learn Beamline Guides APS 14-ID-B (BioCARS)

BEAMLINE GUIDE
APS 14-ID-B (BioCARS)

The Advanced Photon Source
Argonne, IL

The 14-ID-B station is an insertion device station with two in-line undulators (U23 and U27). It supports Laue and time-resolved crystallography, including a serial crystallography approach as well as standard single crystal rotation using MiTeGen MicroLoops with RT capillaries at room temperature, and time-resolved solution scattering. It also supports all standard macromolecular crystallography techniques. 1

APS 14-ID-B Beamline Upgrade Shutdown – Alternative Beamlines

The APS 14-ID-B beamline will be shutting down in 2023 for one year as part of a beamline upgrade project. Listed below are the recommended alternative beamlines. Contact us if you need help in getting ready to conduct your research at one of these alternative beamlines. We can supply you with the tools and instruments you need.

Instructions – Reference the chart below. If the beamline name is hyperlinked that means you can click through to learn more about the alternative beamline such as contact information as well as recommended tools and instruments.

  • Facility – NSLS-II Beamlines

  • SSRL Beamlines

    • Station 4-2

Contacts

Vukica Srajer

Robert Henning

Other Links

Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style N/A N/A
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE, 19 mm, 25 mm
Base Style B1A-Reusable B1A-Reusable, B1-R, B3-R  B5-R
*CryoCrystallography. BioCARS. Retrieved November 4, 2013.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

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