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Learn Beamline Guides DIAMOND I04

BEAMLINE GUIDE
DIAMOND I04

Diamond Light Source
Didcot, Oxfordshire
BART Automounter

I04 is one of seven macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9795 Å (12.658 keV) but it is tuneable over the wavelength range 0.69 – 2.066 Å (6 keV – 18 keV).  On request it is also possible to work at a wavelength as low as 0.62 Å (20keV). 1

Features include:

  • Variable focus beam from 10 x 5 to 110 x 100 µm delivering 3 x 3 x 1011  ph/s at the Se K edge
  • Dectris Eiger2 XE 16M detector
  • Smargon multi-axis goniometer
  • High resolution viewing system
  • Accurate sample centering stages
  • Simple beam visualisation procedures
  • Fine resolution attenuation control
  • DLS BART sample changer – 37 Unipucks / 592 samples
  • Vortex fluorescence detector
  • Automated data processing
  • Remote access

Contacts

Ralf Flaig

Other Links

Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style Uni-Puck Uni-Puck
Mount/Loop Style MicroMounts™ & MicroLoops™ LD

10, 20, 200μm apertures

 Any

10, 20, 200μm apertures
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B5-Reusable B5-R, B-5
*Sample Holders: Cryo-Cooled Samples. Retrieved December 13, 2013

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

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