BEAMLINE GUIDE
CLSI 08ID-1
Canadian Light Source Inc.
Saskatchewan, Canada
ISARA sample changer
The scientific goal of the 08ID-1 beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline consists of white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertical focusing mirror (VFM). The beamline is completed with an innovative endstation and a Pilatus3 S 6M X-ray detector. The beamline controls are based on EPICS and are complemented with MxDC, a user-friendly interface developed in-house.
The source of X-rays on 08ID-1 is a small-gap undulator inserted into the storage ring (and is hence termed an “insertion device”). As its name suggests, the undulator produces a periodic magnetic field that causes the path of the orbiting electrons to undulate, resulting in an emitted X-ray beam of higher spectral brilliance than could be produced by the simpler path taken by the electrons through a bending magnet.
The beamline is equipped with a Röntec XFlash 1001 to perform X-ray spectroscopy measurements for SAD/MAD experiments as well as X-ray fluorescence (XRF) measurements for metal identification. An ISARA automounter allows remote experiments as well as onsite work.1
Contacts
Michel Fodje
Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
| Recommended | Accepted* | |
|---|---|---|
| Puck Style | Uni-Puck | Uni-Puck |
| Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
| Rod Length | 18mm/SPINE | 18mm/SPINE |
| Base Style | B1A-Reusable | B1A-Reusable, B1-Reusable, B3-Reusable, B3S-Reusable |
| *CMCF – Samples and Automounters. Retrieved November 12, 2013. | ||
Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B1A-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
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Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description