BEAMLINE GUIDE
ALS 12.3.1-PX
The Advanced Light Source
Berkeley, CA
SAM (Stanford Automated Mounting system)
The Structurally Integrated Biology for Life Sciences Beamline is located in the Physical Biosciences Division of the Berkley Lawrence National Laboratory.1
Automated Systems at 12.3.1-PX:
The SIBYLS beamline has available a SAM (Stanford Automated Mounting system) style sample mounting robot. We’ve christened it DOMO (Dynamic Offsite MX Operator). Our storage dewar accepts two of the widely available SSRL 96 sample cassettes, allowing you extended uninterrupted beamtime to screen crystals and collect data.2
Contacts
John Tainer, Ph.D
Michal Hammel, Ph.D
Scott Classen, Ph.D
Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
Recommended | Accepted* | |
---|---|---|
Puck Style | SSRL Cassette | SSRL Cassette |
Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
Rod Length | 18mm/SPINE | 18mm/SPINE |
Base Style | B1A-Reusable | B1A-Reusable, B1-R, B3S-R |
*Using the SSRL Automated Mounting (SAM) system |
Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B5-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
-
Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description