LEXSY host T7-TR
Glycerol stocks for constitutive expression.
The Leishmania tarentolae strain T7-TR is used as host strain for inducible LEXSY expression vectors.
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
- Shipping: shipped on dry ice
- Storage Conditions: Store at -80 °C. Avoid freeze/thaw cycles. Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
- Shelf Life: 12 months
- Manufacturers SKU: LT-110
- MiTeGen SKU: M-LT-110
Product Information
- Content: 3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host T7-TR (recombinant Leishmania tarentolae strain expressing bacteriophage T7 RNA polymerase and TET repressor). These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
- Organism: Recombinant Leishmania tarentolae strain constitutively expressing bacteriophage T7 RNA polymerase and TET repressor.
- Biosafety level: 1, Non-pathogenic for mammalians
- Source: Tarentola annularis
- Reactivation of LEXSY host: Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (M-ML-411L) containing the antibiotics Nourseothricin (M-AB-101L) and LEXSY Hygro (M-AB-104L). Incubate at 26 °C and dilute as required.
- Preparation of LEXSY BHI growth medium: Dissolve 37 g/l LEXSY BHI powder (M-ML-412L) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
Immediately before use, add the antibiotics Nourseothricin (M-AB-101L) and LEXSY Hygro. (M-AB-104L) for maintenance of the T7 polymerase and TET repressor genes. - Preparation of glycerol stocks: Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.